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KMID : 1094719970020010033
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.33 ~ p.37
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Some characteristics of cytochromec-555 isolated from sulfide oxidizingXanthomonas sp. DY44
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Cho Kyung-Suk
Park Sang-Jin
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Abstract
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From a heterotrophic bacterium,Xanthomonas sp. DY44 which was previously reported to oxidize hydrogen sulfide (H2S) to polysulfide, cytochromec-555 (cyt.c-555) responsible for oxidation of sulfide was purified by DEAE-Toyopearl and Sepadex G-75 column chromatography. Cyt.c-555 with a molecular weight of 12,500 showed maximum absorption at 555 nm (α-peak), 522 nm (β-peak) and 417 nm (γ-peak) for the reduced form which was prepared by addition of Na2S2O4. Cyt.c-555 was also reduced by addition of sulfide (Na2S and H2S), and the oxidized products of sulfide by cyt.c-555 was identified as polysulfide. The reduced form of cyt.c-555 was suggested to be oxidized coupled with cyt.c oxidase which is tolerant to sulfide.
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KEYWORD
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Xanthomonas sp., cytochrome, hydrogen sulfide, oxidation
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